288

19.24

19.25

­

Other useful databases are SMART (Simple Modular Architecture Research Tool; for

annotation, search by name or sequence), Pfam or ProDom (Protein-Domain; based on

consensus or multiple alignment of a protein or DNA sequence), please inform yourself on

the corresponding pages.

In addition, domains and functional motifs (based on SMART and Pfam) of eukaryotic

proteins can be identified with the ELM server (Eucaryotic Linear Motif), which can also

predict signal peptides and provide information on secondary structure. Alternatively, one

can also perform a multiple alignment of several sequences oneself in order to find domains

or conserved regions and specific differences. For this purpose, the already mentioned

software MUSCLE is a useful tool (see tutorial section phylogenetic tree). It can be used

online on the EBI website (https://www.ebi.ac.uk/Tools/msa/muscle/), alternatively it can

be installed and used locally (https://www.drive5.com/muscle/).

19  Tutorial: An Overview of Important Databases and Programs